Synthesis chemica DNA in solido-phasi synthesi belli et phosphoramidite chemiae militaris fundatur.Differt a synthesi biologico DNA, materia in synthesi chemica DNA est DMT (4, 4-dimethoxytrityl) et phosphoramidite deoxyriboxyside mutata, ut in Figura 1. DNA synthesis exercetur in solido auxilio (variis offerre possumus.Oligo Synthesis Columna) , ie CPG (polystyrenum po- sterium) et PS (polystyrenum) et tota synthesis in a.DNA/RNA synthesizer, quod est instrumentum nostrum principale, exemplar diversum habet ut: HY Singulus Channel Synthesizer, HY-12, HY-192 et etc. synthesis cycli.Synthesis cycli typice continet quattuor gradus, Deprotectionem, Copulam, Cappingem et Oxidationem (Figura II).Deprotectio ad removendum coetus DMT in solido auxilio vel hydroxyli coetus 5' in nucleoside priore, 3% acidum trichloroaceticum in dichloromethane ponitur pro deprotectione reagens.Tum phosphoramidite deoxyriboxysidum modificatum permissum est ut exposita coetus hydroxyli ope activatoris, id est 5-ethylthiotetrazoli seu 4, 5-dicyanoimidazoli, phosphitetriester (III) formans et coniunctionis gradum intellexit.Gradus Capping exercetur ad turbationem hydroxyli unreacted in copulatione gressu interclusam, ad minuendum sequentium defectionis necopinatum formationem.Denique instabilis phosphitetriester (III) oxidized ad phosphortriester chemicum firmum (V) cum iodine oxidantis in praesenti pyridine.
Synthesisata DNA cohaerere potuit a solido auxilio ab aminolysi, cyanoethyl 2-coethei in phosphortriester et inter nucleobasi munitus, simul adglutinatur, synthesis laminarum et synthesis columnarum in eculeis directe in camera reactionis collocantur. deDeprotection Equipment.Cruda DNA praeparari potuerunt ab HPC, electrophoresi et OPC in postulatis, etiam tibi providere possumusPurificatio Equipmentfor Post-processus.
Figure 1. De chemicis structura dABzphosphoramidite.
Figure 2. Mechanismus synthesis chemicae DNA.
Post tempus: Aug-09-2022